Gene Expression Signatures in Tree Shrew Sclera in Response to Three Myopiagenic Conditions
نویسندگان
چکیده
منابع مشابه
Gene expression signatures in tree shrew choroid in response to three myopiagenic conditions
We examined gene expression in tree shrew choroid in response to three different myopiagenic conditions: minus lens (ML) wear, form deprivation (FD), and continuous darkness (DK). Four groups of tree shrews (n=7 per group) were used. Starting 24 days after normal eye opening (days of visual experience [DVE]), the ML group wore a monocular -5D lens for 2 days. The FD group wore a monocular trans...
متن کاملAlterations in protein expression in tree shrew sclera during development of lens-induced myopia and recovery.
PURPOSE During the development of, and recovery from, negative lens-induced myopia there is regulated remodeling of the scleral extracellular matrix (ECM) that controls the extensibility of the sclera. Difference gel electrophoresis (DIGE) was used to identify and categorize proteins whose levels are altered in this process. METHODS Two groups of five tree shrews started monocular lens wear 2...
متن کاملDifferential protein expression in Tree Shrew sclera during development of lens-induced myopia and recovery
PURPOSE The tree shrew model of refractive development is particularly useful because, like humans, tree shrews have a fibrous sclera. Selective changes in some candidate extracellular matrix proteins and mRNAs have been found in the sclera during the development of and recovery from induced myopia. We undertook a more neutral proteomic analysis using two-dimensional gel electrophoresis and mas...
متن کاملPatterns of mRNA and protein expression during minus-lens compensation and recovery in tree shrew sclera
PURPOSE To increase our understanding of the mechanisms that remodel the sclera during the development of lens-induced myopia, when the sclera responds to putative "go" signals of retinal origin, and during recovery from lens-induced myopia, when the sclera responds to retinally-derived "stop" signals. METHODS Seven groups of tree shrews were used to examine mRNA levels during minus lens comp...
متن کاملA Novel Organ Culture Model to Quantify Collagen Remodeling in Tree Shrew Sclera
Increasing evidence suggests that unknown collagen remodeling mechanisms in the sclera underlie myopia development. We are proposing a novel organ culture system in combination with two-photon fluorescence imaging to quantify collagen remodeling at the tissue- and lamella-level. Tree shrew scleral shells were cultured up to 7 days in serum-free media and cellular viability was investigated unde...
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ژورنال
عنوان ژورنال: Investigative Opthalmology & Visual Science
سال: 2013
ISSN: 1552-5783
DOI: 10.1167/iovs.13-12551